A Stride in DNA Break Analysis
A Stride in DNA Break Analysis
STRIDE
SensiTive Recognition
of Individual DNA Ends
STRIDE is the first platform technology for the direct detection of DNA ends. It outcompetes all other tools in its sensitivity, providing a reliable measure of DNA damage at an individual lesion level. STRIDE selectively marks either single- or double-strand DNA breaks. It tracks even subtle elevations in DNA break levels, which translates into a better understanding of drug pharmacodynamic effects, and their molecular mechanisms of action.
The exceptional qualities of our technology are derived from direct fluorescent labelling of free DNA ends, strong signal amplification, and eliminating any nonspecific background.
STRIDE is a versatile method that enables selective DNA break detection in cell nuclei or DNA fragments in the cytoplasm. The labelling can be combined with immunofluorescent staining of various DNA repair process markers, signalling proteins and other subcellular structures. The STRIDE assay itself can be personalised to provide a reliable tool for the analysis of any DNA repair pathway of interest.
Currently At
DNA Damage Response
STRIDE, with its sensitivity and precision, provides direct measure of DNA breaks, independently of repair processes
Nuclear Medicine
STRIDE selectively marks single- and double-strand DNA breaks to provide insight on mechanisms of action
Immunotherapy
STRIDE detects cytosolic DNA fragments to measure drugs’ acute effect and the immune response of cells
Where DNA Breaks
Our Insight Begins
Applications
Labelling with STRIDE can be performed on a variety of biological material such as fixed cultured cells, frozen and formalin-fixed paraffin-embedded tissue sections, isolated blood cells, tissue microarrays, germ cells, blood smears and can be adapted to new biological material.
Our Process
We approach DNA break detection end-to-end, i.e. from laboratory cell work and tissue preparation, performing the assay and microscopy imaging, to data analysis with our automated machine learning based approach, and comprehensive result interpretation. With our input, our partners get broader insight into their drugs’ mechanism of action, efficiency, pharmacodynamics and safety.
Scalability
STRIDE is a game-changer in any domain where DNA integrity matters. It can be applied in many fields to assess off-target effects or screen genetic material quality (such as in gene editing, reproductive medicine and agriculture among many others). From a technological point of view, since STRIDE is based on fluorescence, it can be scaled to various detection strategies, such as fluorescence and confocal microscopy, flow cytometry or high-throughput screening. It also has potential to be established as a predictive biomarker and a companion diagnostic in targeted cancer therapies.